HGP-write:
Testing Large Genomes in Cells

Hosted by: Jef Boeke, George Church, Andrew Hessel and Nancy J Kelley

8:30 – 9:00 Registration and Continental Breakfast

9:00 – 9:10 Welcome to Harvard Medical School

• Overview of the Meeting, George Church and Nancy J Kelley

9:10 – 10:00 Overview of Project and Current Synthetic Genomics Environment

• Overview of HGP-write, Jef Boeke, Andrew Hessel
• Lessons Learned: Sc 2.0: The Synthetic Yeast Genome Project, Leslie Mitchell, Patrick Cai
• Lessons Learned: From rEcoli to 1 Million Genomes, George Church

10:00 – 10:50 HGP-write: Presentation/Discussion of 1% Pilot Projects

• Jef Boeke / Farren Isaacs  |  UltraSafe Cell Line
• Harris Wang  |  Synthesizing a Prototrophic Human Genome
• Liam Holt  |  The Seven Signals Toolbox: Enabling HGP-write Cell and Tissue Therapies
• Nili Ostrov  |  Recoding E.coli to 57 Codons

10:50 – 11:20 Break

11:10 – 12:20 Related Research Areas That Could Complement or Benefit HGP-write

• Alex Ng  |  Human Organoids, iPS Differentiation
• Luhan Yang  |  Transplantation
• Vladimir Larionov  |  Human Artificial Chromosomes
• Alina Chan  |  De Novo Chromosomes the Shuttle Between Yeast and Mammalian Systems
• Timothy Lu  |  Microbiome  Engineering
• Amy Wagers  |  Gene Therapy
• Dieter Egli  |  Haploid Embyonic Stem Cells

12:20 – 1:40 Panel/Group Discussion | Lunch (provided)

• Is “HGP-write: Testing Large Genomes in Cells” a wise choice?
• Moderator: Marc Vidal, Harvard Medical School Department of Genetic
  • Pilar Ossario, University of Wisconsin Madison
  • Debra Methews, Johns Hopkins Berman Institute of Bioethics
  • Scott Fahrenkrug, Recombinetics
  • Nicole Lockhart, National Human Genome Research Institute

1:40 – 2:00 Safety Engineering

• Farren Isaacs  |  General Introduction
• Jim Collins  |  Building Genetic Containment

2:00 – 2:30 Scientific Talks on Infrastructure, Technology Development and Technical Challenges

• Sean Stevens  |  Synthetic Genomics, Inc.
• Helge Bastian  |  Thermo Fisher Scientific
• Huimin Zhao  |  Fully Automated Genome-Scale Engineering

2:30 – 3:30 Industry Engagement Panel

• Moderator: Andrew Hessel  |  Autodesk
  • Emily Leproust  |  Twist Biosciences
  • Kevin Munnelly  |  Gen 9
  • Sandra Glucksmann  |  Editas
  • Brian Zambrowicz  |  Regeneron

3:30 – 4:15 Break

4:15 – 5:15 Regulatory and Bioethical Implications of the 1% Pilot Projects (Panel Discussion)

• Moderator: Eleonore Pauwels, Wilson Center
  • Jeantine Lunshof  |  Harvard Medical School
  • Pilar Ossorio  |  University of Wisconsin Madison
  • Matthew Porteus  |   Stanford University
  • Barbara Evans  |  University of Houston Law Center
  • Nicole Lockhart  |  National Human Genome Research Institute

5:15 – 5:45 Wrap Up and Next Steps (Nancy J Kelley)

6:00 – 8:00 Evening Cocktails